Improved Acoustic Differential Extraction on a Microdevice for Separation of Sperm Cells and Epithelial Cell Lysate
نویسندگان
چکیده
Genetic analysis of samples obtained from sexual assault evidence relies on separation of male and female components of the recovered genetic material. One separation technique, acoustic differential extraction (ADE) on a microfluidic device involving acoustic trapping of sperm cells in the presence of female lysate, has previously been demonstrated. However, laminar flow valving (LFV) utilized to direct the sperm and lysate to different outlets was problematic, as a male DNA profile was not obtained. The presented work describes improvements to the ADE technique to enable efficient separation of mixed biological samples, resulting in independent male and female DNA profiles.
منابع مشابه
Bead-assisted Acoustic Differential Extraction of Sperm Cells in Dilute Samples for Potential Forensic Analyses
Here we describe an improved method of separating sperm cells from lysed epithelial cells via acoustic cell trapping. In particular, we show that in the processing of samples with low numbers of sperm cells, addition of polymeric beads to the sample[1] allowed for better performance in terms of higher trapping efficiency versus our previous acoustic differential extraction (ADE) results[2]. We ...
متن کاملSeparation of sperm and epithelial cells in a microfabricated device: potential application to forensic analysis of sexual assault evidence.
Forensic DNA analysis of sexual assault evidence requires separation of DNA from epithelial (victim) and sperm (perpetrator) cells. The conventional method used by crime laboratories, which is termed "differential extraction", is a time-consuming process. To supplant the conventional process, separation of sperm from a biological mixture containing epithelial cells has been demonstrated on a mi...
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The proven utility of forensic DNA evidence has increased the demand for DNA analysis services. Although conventional DNA analysis techniques are effective, they are time-consuming and laborious, which contributes to the overall cost and to an overwhelming backlog of forensic casework samples with possible biological evidence. Research efforts have focused on the development of more rapid and e...
متن کاملBoston University
Biological mixtures comprised of multiple individuals’ tissues or bodily fluids are commonly encountered and gathered during crime scene evidence collection. Sexual assault samples are one such mixture, usually comprised of epithelial cells and sperm. Sexual assault mixtures may contain a large amount of non-sperm cells in comparison to sperm cells. In such instances, it is imperative to separa...
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